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ABSTRACT

Kumpulan Jurnal Pemurnian Zat Pdf - DownloadKumpulan Jurnal Pemurnian Zat Pdf – PURIFICATION AND CHARACTERIZATION OF INHIBITOR PROTEASE FROM Chromohalobacter sp. 6A3, BACTERIES THAT BASED WITH SPONS Xetospongia testudinaria [Purification and Characterization of Protease Inhibitors from Chromohalobacter sp. 6A3, Bacteria-associated with Sponge Xetospongia testudinaria] Tati Nurhayati1), Maggy T. Suhartono2), Lilis Nuraida2), Sri Budiarti Poerwanto3) 1) Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University 2) Department of Science and Food Technology, Faculty of Agricultural Technology, Bogor Agricultural University 3) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University Received November 26, 2009 / Approved December 15, 2010 ABSTRACT Various sponges has been reported to produce protease inhibitors which could inhibit the protease activity of pathogenic bacteria. The previous research showed that bacteria-associated with sponge could produce bioactive compounds similar to their host, including protease inhibitors. The purposes of this research were to purify protease inhibitors from Chromohalobacter sp. 6A3 and to study the characteristics of protease inhibitors. The result shows that the protein can be extracted by 30% (v / v) acetone, purified by gel filtration (Sephadex G-75) and finally, purified by anion exchanger (Sephadex A-50). The optimum temperature and pH were 30 ° C and 5 respectively. The protease inhibitor can resist heating at 30 oC for 10 minutes. Incubation of the inhibitor at 30 oC, pH 5, still retained its activity until 3 hours. The purified enzyme inhibitor was also still active after incubated at pH from 5 to 6 for 1 hour. The most susceptible substrate (enzyme) for the inhibitor was protease from P. aeruginosa. The protease inhibitor was inhibited by metal ions except Na + 1mM. Activity of the inhibitor increased twofold by SDS 5 mM. IC 50 of the protease inhibitor was 3.48 nM. The protease inhibitor inhibited the enzyme uncompetitively. Key words: chromohalobacter, protease inhibitor, sponge.

  1. PEMURNIAN DAN KARAKTERISASI INHIBITOR PROTEASE DARI Chromohalobacter sp. 6A3, BAKTERI YANG BERASOSIASI DENGAN SPONS Xetospongia testudinaria [Purification and Characterization of Protease Inhibitor from Chromohalobacter sp. 6A3, Bacteria-associated with S
  2. OPTIMASI PROSES DEASIDIFIKASI DALAM PEMURNIAN MINYAK SAWIT MERAH SKALA PILOT PLANT [Optimization of Deacidification Process in Red Palm Oil Purification on Pilot Plant Scale]
  3. PEMURNIAN PROTEASE DARI BUAH DAN DAUN MENGKUDU (Morinda citrifolia L.) [Purification of Proteases from Fruits and Leaves of Noni (Morinda citrifolia L.)]
  4. OPTIMASI PEMURNIAN ASAP CAIR DENGAN METODA REDISTILASI [Optimation of Liquid Smoke Purification by Redistilation Method)
  5. TEKNOLOGI PEMURNIAN SENYAWA DENGAN METODA KROMATOGRAFI
  6. PROSES PEMURNIAN YELLOW CAKE DARI LIMBAH PABRIK PUPUK
  7. Pemurnian Biogas Dari Gas Pengotor Hidrogen Sulfida (H2S) Dengan Memanfaatkan Limbah Geram Besi Proses Pembubutan
  8. Pemurnian Kondensat Hasil Pembuatan Gula Aren (Arenga pinnata) dengan Menggunakan Arang Aktif
  9. APLIKASI TEKNOLOGI REVERSE OSMOSIS UNTUK PEMURNIAN AIR SKALA RUMAH TANGGA
  10. PEMURNIAN NATRIUM ALGINAT DARI Sargassum duplicatum J.G. Agardh, Turbinaria decurrens (Bory) DAN Turbinaria ornata (Turner) J. Argardh ASAL PERAIRAN TERNATE, MALUKU UTARA

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